Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 WG914 (LMBP 12434)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | WG914 |
| Accession number: | LMBP 12434 |
| Depositor: | This host strain was deposited by Dr D.E. Culham1 and Prof. Em. Dr J.M. Wood1. 1 Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | LB-Lennox |
| Selection marker: | - |
| Temperature: | 37°C |
| Genotype: | F- trp lacZ rpsL thi ∆putPA101 proU205 ∆proQ676 |
| Phenotype: | StrR |
| Properties: | - |
| Further information: | This host strain was created by inserting an in-frame chromosomal proQ deletion (∆proQ676) into E. coli K12 WG210. Resistance to streptomycin has been experimentally confirmed by BCCM/GeneCorner. |
| Original reference: | Smith et al., Biochemistry 43 (2004), 12979-12989 [PMID: 15476391] [DOI: 10.1021/bi048561g] |
| Restricted use: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department. |
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Refer in your Materials and Methods: |
Escherichia coli K12 WG914 (LMBP 12434) is available at BCCM/GeneCorner. It was deposited by Dr D.E. Culham and Prof. Em. Dr J.M. Wood and was published in Smith et al., 2004. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.